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The extraction process and proposed mechanisms for GPEVs in treating <t>UVB-induced</t> Skin aging. Extracellular vesicles derived from Gynostemma pentaphyllum (GPEVs), isolated and purified from fresh whole herb via differential ultracentrifugation, exhibit anti-photoaging properties. <t>UVB</t> <t>radiation</t> triggers the up-regulation of STING, activating the TBK1-CTCF pathway and causing photoaging. GPEVs effectively promote STING degradation, reducing CTCF-mediated aging gene transcription, protecting against UVB-induced skin aging.
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The extraction process and proposed mechanisms for GPEVs in treating <t>UVB-induced</t> Skin aging. Extracellular vesicles derived from Gynostemma pentaphyllum (GPEVs), isolated and purified from fresh whole herb via differential ultracentrifugation, exhibit anti-photoaging properties. <t>UVB</t> <t>radiation</t> triggers the up-regulation of STING, activating the TBK1-CTCF pathway and causing photoaging. GPEVs effectively promote STING degradation, reducing CTCF-mediated aging gene transcription, protecting against UVB-induced skin aging.
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The extraction process and proposed mechanisms for GPEVs in treating <t>UVB-induced</t> Skin aging. Extracellular vesicles derived from Gynostemma pentaphyllum (GPEVs), isolated and purified from fresh whole herb via differential ultracentrifugation, exhibit anti-photoaging properties. <t>UVB</t> <t>radiation</t> triggers the up-regulation of STING, activating the TBK1-CTCF pathway and causing photoaging. GPEVs effectively promote STING degradation, reducing CTCF-mediated aging gene transcription, protecting against UVB-induced skin aging.
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Peschl Ultraviolet 150 w medium pressure mercury lamp
| a) 3D model of the photocatalytic screening reactor (left) and cross-section (right): 1) cooling tube; 2) irradiation vessel with photocatalytic membrane; 3) central UV filter reservoir; 4) irradiation vessel cage; 5) spectrometer optics mount; 6) stir bar; 7) mounting column; 8) timing belt (GT2, 6 mm wide); 9) pulley (GT2, 5 mm inner diameter, 21 teeth); 10) ball bearing (6 mm inner diameter); 11) stepper motor (NEMA 17); 12) b) cross section of the reactor: connector for stir plate control; 13) light source (150 W Hg medium-pressure lamp); 14) mount for the central UV filter reservoir; 15) microcontroller unit (MCU), Arduino Uno with CNC shield and stepper driver. c) Photograph of the reactor system.
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Osram Sylvania mr16 halogen lamp cup
| a) 3D model of the photocatalytic screening reactor (left) and cross-section (right): 1) cooling tube; 2) irradiation vessel with photocatalytic membrane; 3) central UV filter reservoir; 4) irradiation vessel cage; 5) spectrometer optics mount; 6) stir bar; 7) mounting column; 8) timing belt (GT2, 6 mm wide); 9) pulley (GT2, 5 mm inner diameter, 21 teeth); 10) ball bearing (6 mm inner diameter); 11) stepper motor (NEMA 17); 12) b) cross section of the reactor: connector for stir plate control; 13) light source (150 W Hg medium-pressure lamp); 14) mount for the central UV filter reservoir; 15) microcontroller unit (MCU), Arduino Uno with CNC shield and stepper driver. c) Photograph of the reactor system.
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| a) 3D model of the photocatalytic screening reactor (left) and cross-section (right): 1) cooling tube; 2) irradiation vessel with photocatalytic membrane; 3) central UV filter reservoir; 4) irradiation vessel cage; 5) spectrometer optics mount; 6) stir bar; 7) mounting column; 8) timing belt (GT2, 6 mm wide); 9) pulley (GT2, 5 mm inner diameter, 21 teeth); 10) ball bearing (6 mm inner diameter); 11) stepper motor (NEMA 17); 12) b) cross section of the reactor: connector for stir plate control; 13) light source (150 W Hg medium-pressure lamp); 14) mount for the central UV filter reservoir; 15) microcontroller unit (MCU), Arduino Uno with CNC shield and stepper driver. c) Photograph of the reactor system.
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Philips Healthcare hpl n 125 w lamp
| a) 3D model of the photocatalytic screening reactor (left) and cross-section (right): 1) cooling tube; 2) irradiation vessel with photocatalytic membrane; 3) central UV filter reservoir; 4) irradiation vessel cage; 5) spectrometer optics mount; 6) stir bar; 7) mounting column; 8) timing belt (GT2, 6 mm wide); 9) pulley (GT2, 5 mm inner diameter, 21 teeth); 10) ball bearing (6 mm inner diameter); 11) stepper motor (NEMA 17); 12) b) cross section of the reactor: connector for stir plate control; 13) light source (150 W Hg medium-pressure lamp); 14) mount for the central UV filter reservoir; 15) microcontroller unit (MCU), Arduino Uno with CNC shield and stepper driver. c) Photograph of the reactor system.
Hpl N 125 W Lamp, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The extraction process and proposed mechanisms for GPEVs in treating UVB-induced Skin aging. Extracellular vesicles derived from Gynostemma pentaphyllum (GPEVs), isolated and purified from fresh whole herb via differential ultracentrifugation, exhibit anti-photoaging properties. UVB radiation triggers the up-regulation of STING, activating the TBK1-CTCF pathway and causing photoaging. GPEVs effectively promote STING degradation, reducing CTCF-mediated aging gene transcription, protecting against UVB-induced skin aging.

Journal: Bioactive Materials

Article Title: Gynostemma pentaphyllum -derived extracellular vesicles alleviate skin aging by destabilizing STING

doi: 10.1016/j.bioactmat.2026.03.010

Figure Lengend Snippet: The extraction process and proposed mechanisms for GPEVs in treating UVB-induced Skin aging. Extracellular vesicles derived from Gynostemma pentaphyllum (GPEVs), isolated and purified from fresh whole herb via differential ultracentrifugation, exhibit anti-photoaging properties. UVB radiation triggers the up-regulation of STING, activating the TBK1-CTCF pathway and causing photoaging. GPEVs effectively promote STING degradation, reducing CTCF-mediated aging gene transcription, protecting against UVB-induced skin aging.

Article Snippet: The UVB radiation source consists of four UVB lamps (Philips, Shanghai, China) with a peak intensity at 311 nm.

Techniques: Extraction, Derivative Assay, Isolation, Purification

| a) 3D model of the photocatalytic screening reactor (left) and cross-section (right): 1) cooling tube; 2) irradiation vessel with photocatalytic membrane; 3) central UV filter reservoir; 4) irradiation vessel cage; 5) spectrometer optics mount; 6) stir bar; 7) mounting column; 8) timing belt (GT2, 6 mm wide); 9) pulley (GT2, 5 mm inner diameter, 21 teeth); 10) ball bearing (6 mm inner diameter); 11) stepper motor (NEMA 17); 12) b) cross section of the reactor: connector for stir plate control; 13) light source (150 W Hg medium-pressure lamp); 14) mount for the central UV filter reservoir; 15) microcontroller unit (MCU), Arduino Uno with CNC shield and stepper driver. c) Photograph of the reactor system.

Journal: MethodsX

Article Title: Development of a photocatalytic membrane screening reactor (PMSR) for standardized evaluation of immobilized photocatalytic support materials

doi: 10.1016/j.mex.2026.103938

Figure Lengend Snippet: | a) 3D model of the photocatalytic screening reactor (left) and cross-section (right): 1) cooling tube; 2) irradiation vessel with photocatalytic membrane; 3) central UV filter reservoir; 4) irradiation vessel cage; 5) spectrometer optics mount; 6) stir bar; 7) mounting column; 8) timing belt (GT2, 6 mm wide); 9) pulley (GT2, 5 mm inner diameter, 21 teeth); 10) ball bearing (6 mm inner diameter); 11) stepper motor (NEMA 17); 12) b) cross section of the reactor: connector for stir plate control; 13) light source (150 W Hg medium-pressure lamp); 14) mount for the central UV filter reservoir; 15) microcontroller unit (MCU), Arduino Uno with CNC shield and stepper driver. c) Photograph of the reactor system.

Article Snippet: The light source (13) is placed inside the cooling jacket; in the configuration used here, a 150 W medium-pressure mercury lamp (TQ150, Peschl Ultraviolet GmbH) was employed.

Techniques: Irradiation, Membrane, Control

| Emission spectra of a 150 W mercury medium-pressure lamp, normalized to the maximum intensity (100 %), under different filter conditions: a) no filter, b) borosilicate glass (cut-off at λ=295 nm), c) nitrate solution (cut-off at λ=325 nm), d) nitrite solution (cut-off at λ=405 nm). Hatched area shows the gap which was characterized by chemical actinometry.

Journal: MethodsX

Article Title: Development of a photocatalytic membrane screening reactor (PMSR) for standardized evaluation of immobilized photocatalytic support materials

doi: 10.1016/j.mex.2026.103938

Figure Lengend Snippet: | Emission spectra of a 150 W mercury medium-pressure lamp, normalized to the maximum intensity (100 %), under different filter conditions: a) no filter, b) borosilicate glass (cut-off at λ=295 nm), c) nitrate solution (cut-off at λ=325 nm), d) nitrite solution (cut-off at λ=405 nm). Hatched area shows the gap which was characterized by chemical actinometry.

Article Snippet: The light source (13) is placed inside the cooling jacket; in the configuration used here, a 150 W medium-pressure mercury lamp (TQ150, Peschl Ultraviolet GmbH) was employed.

Techniques: